PCR primer pair and application thereof

US11739319B2 · kind B2 · utility

Assignee

• MGI Tech Co., Ltd. · CN

Inventors

• Lin Yang · Shanghai, CN
• Guodong Huang · Lo Wu, CN
• Ya Gao · Hubei, CN
• Yanyan Zhang · Lo Wu, CN
• Haiping Zhang · Tangxia, CN
• Fang Chen · Lo Wu, CN
• Haojun Jiang · Lo Wu, CN
• Shaohong Zhang · Lo Wu, CN
• Yuqian Wang · Longbeilingcun, CN
• Yifan Xie · Lo Wu, CN
• Hui Jiang · Lo Wu, CN

Key dates

• Filing date: Jun 20, 2017
• Grant date: Aug 29, 2023
• Expiry date: Mar 4, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.