Compositions and methods for genomic DNA and gene expression analysis in single cells
US11739321B2 · kind B2 · utility
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24Claims
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Key dates
| Filing date | Jan 7, 2020 |
| Grant date | Aug 29, 2023 |
| Priority date | — |
| Expiry date | Feb 5, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07049
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
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