Recombinant microorganism for producing citicoline and method for producing citicoline
US11739357B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 7, 2020 |
| Grant date | Aug 29, 2023 |
| Priority date | — |
| Expiry date | Jul 6, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y306/01026
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a recombinant microorganism for producing citicoline and a method for producing citicoline by using the recombinant microorganism, wherein genes for degradation and utilization of citicoline, choline, and phosphocholine are knocked out, In addition, a pyrimidine nucleoside synthesis pathway is genetically engineered to remove feedback inhibition to the synthesis pathway. A yield of more than 20 g/L of citicoline can be obtained with recombinant strains in a 5-liter fermenter by means of a biological fermentation method, achieving industrial mass production with low citicoline production costs and less pollution; therefore, the method is a simple, environmentally friendly and has a relatively high promotion and application value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.