Digital amplification with primers of limited nucleotide composition
US11739375B2 · kind B2 · utility
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33Claims
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Assignee
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Key dates
| Filing date | Aug 10, 2018 |
| Grant date | Aug 29, 2023 |
| Priority date | — |
| Expiry date | May 15, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/159
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of digital amplification using primers of limited nucleotide composition. Limited nucleotide composition means that the primers are underrepresented in at least one nucleotide type. Such primers have much reduced capacity to prime from each other or to extend initiated by mispriming from other than at their intended primer binding sites in a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.