Methods of cloning nucleic acids or producing proteins in a low endotoxin organism
US11746321B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Mar 25, 2021 |
| Grant date | Sep 5, 2023 |
| Priority date | — |
| Expiry date | Apr 29, 2041 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides engineered Vibrio sp. organisms that comprise a genetic modification to either or both of the lpxL and/or lpxM genes. The organisms score substantially lower in an in vitro endotoxin assay versus the unmodified or wild type organism. The organisms preserve substantially the growth rate of the corresponding unmodified organisms. The organisms can also have an exogenous nucleic acid cloned in the organism, or an exogenous nucleic acid encoding a protein, polypeptide, or peptide expressed by the organism, and optionally secreted from the organism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.