Performance-enhanced and storage stable protease variants

US11746341B2 · kind B2 · utility

Assignee

• Henkel AG & Co. KGaA · DE

Inventors

• Christian Degering · Erkrath, DE
• Layla Fernandez · Cologne, DE
• Sabine Griemert · Monheim am Rhein, DE
• Nina Mussmann · Willich, DE
• Inken Prueser · Dressendorf, DE
• Daria Strauss · Dressendorf, DE
• Susanne Wieland · Nievenheim, DE

Key dates

• Filing date: Nov 28, 2019
• Grant date: Sep 5, 2023
• Expiry date: Mar 26, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/21062
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Proteases may include an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and has (i) one or more first amino acid substitutions at positions corresponding to positions 3, 4, 99, 199, or combinations thereof, and (ii) one or more second amino acid substitutions at positions corresponding to positions 9, 21, 42, 44, 105, 112, 113, 131, 137, 139, 141, 145, 159, 168, 176, 177, 182, 193, 198, 204, 205, 206, 210, 212, 230, 234, 250, 253, 255, 259, 267, or combinations thereof based on the numbering according to SEQ ID NO:1, and relates to the preparation and use thereof. Proteases of this kind demonstrate very good stability with good cleaning performance.