Saccharomyces cerevisiae strain with high yield of ethyl butyrate and construction method and application of Saccharomyces cerevisiae strain

US11746353B2 · kind B2 · utility

Assignee

• Tianjin University of Science and Technology · CN

Inventors

• Yefu Chen · Tianjin, CN
• Yanrui Ma · Tianjin, CN
• Yongjing Du · Tianjin, CN
• Sen Jiang · Tianjin, CN
• Jinying Ren · Tianjin, CN
• Guo Zhang · Tianjin, CN
• Xinyue Kang · Tianjin, CN
• Peng Zheng · Tianjin, CN
• Xiaole Wu · Tianjin, CN
• Dongguang Xiao · Tianjin, CN
• Xuewu Guo · Tianjin, CN

Key dates

• Filing date: Dec 25, 2020
• Grant date: Sep 5, 2023
• Expiry date: Aug 5, 2041

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.