Methods and platform for screening and selecting metabolites and their receptors

US11746374B2 · kind B2 · utility

Assignees

• The Board of Regents of the University of Texas System · US
• THE TEXAS A&M UNIVERSITY · US

Inventors

• Andrew Ellington · Austin, US
• Jimmy Gollihar · Austin, US
• Katy KAO · College Station, US
• Elizabeth GARDNER · Austin, US

Key dates

• Filing date: Jun 15, 2018
• Grant date: Sep 5, 2023
• Expiry date: Jan 24, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods and platforms using Receptor Compartmentalized Partnered Replication (CPR), in which the partner gene is a receptor, signal transduction pathway, or metabolic pathway that leads to the production of an effector molecule for the receptor or signal transduction pathway. The signal transduction pathway or receptor is coupled to the production of a thermostable polymerase. Emulsification of libraries of organisms with primers that can amplify the partner gene led to the selective amplification of those partner genes that were best able to produce the thermostable polymerase during thermal cycling of the emulsion.