Methods for amplification of cell-free DNA using ligated adaptors and universal and inner target-specific primers for multiplexed nested PCR

US11746376B2 · kind B2 · utility

Assignee

• Natera, Inc. · US

Inventors

• Matthew Rabinowitz · San Francisco, US
• Matthew Hill · Belmont, US
• Bernhard Zimmermann · Manteca, US
• Johan Baner · San Francisco, US
• George Gemelos · Walnut, US
• Milena Banjevic · Los Altos Hills, US
• Allison Ryan · Redwood City, US
• Styrmir Sigurjonsson · San Jose, US
• Zachary Demko · San Francisco, US

Key dates

• Filing date: Feb 20, 2020
• Grant date: Sep 5, 2023
• Expiry date: Feb 20, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.