Triple-internal standard based glycan structural assignment method for capillary electrophoresis analysis of carbohydrates
US11747271B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 17, 2017 |
| Grant date | Sep 5, 2023 |
| Priority date | — |
| Expiry date | May 10, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8836
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.