Methods and compositions for analyte quantification

US11754536B2 · kind B2 · utility

Assignee

• MATTERWORKS INC · US

Inventors

• Timothy Kassis · Saugus, US
• Jefferson Pruyne · Somerville, US
• Mark David Simon · Somerville, US
• Mimoun Cadosch Delmar Akerman · Brookline, US
• Jennifer Campbell · Arlington, US
• Ana HENRIQUES DA COSTA · Cambridge, US
• Laura Kolinsky · Boston, US
• John M. Geremia · Watertown, US

Key dates

• Filing date: Sep 7, 2022
• Grant date: Sep 12, 2023
• Expiry date: Sep 7, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/045
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed are methods, libraries, and samples for quantifying a target analyte in a laboratory sample including the target analyte. The methods typically include the step of estimating the amount of the target analyte in the laboratory sample from mass spectrometric data including signal intensities for the target analyte and one or more internal standards, where the mass spectrometric data are an output of a mass spectrometric analysis of a target sample produced from the laboratory sample and a predetermined amount of the one or more internal standards. The present disclosure also provides a method for analyte quantification. The method comprises adding one or more calibrators to a sample comprising one or more analytes; applying mass spectrometry (MS) to the sample; and using a trained machine learning model to determine an absolute concentration of the one or more analytes.