CRISPR protein inhibitors

US11760984B2 · kind B2 · utility

Inventors

• Amit Choudhary · Boston, US
• Kurt Cox · Cambridge, US
• Basudeb Maji · Boston, US
• Peng Wu · New Rochelle, US
• Hari K. K. Subramanian · San Diego, US
• Elisa Franco · Oakland, US

Key dates

• Filing date: Oct 31, 2018
• Grant date: Sep 19, 2023
• Expiry date: Jul 12, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The embodiments disclosed herein utilize fluorescence polarization based preliminary screen to identify a putative set of Cas inhibitors from an initial set of candidate inhibitors. The primary screening assay is followed by secondary screening assay to validate the putative set of inhibitors selected by the preliminary screen. In some embodiments, the present disclosure includes compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.