PCR primer pair and application thereof

US11760995B2 · kind B2 · utility

Assignee

• MGI Tech Co., Ltd. · CN

Inventors

• Lin Yang · Shanghai, CN
• Ya Gao · Hubei, CN
• Guodong Huang · Lo Wu, CN
• Yicong Wang · Seattle, US
• Yuqian Wang · Longbeilingcun, CN
• Yanyan Zhang · Lo Wu, CN
• Fang Chen · Lo Wu, CN
• Na Zhong · Lo Wu, CN
• Hui Jiang · Lo Wu, CN
• Xun Xu · Shanghai, CN

Key dates

• Filing date: Jun 20, 2017
• Grant date: Sep 19, 2023
• Expiry date: May 12, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3′ end of the first primer, and the first random sequence is located on 5′ end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3′ end of the second primer, and the second random sequence is located on 5′ end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.