Soybean genetic transformation method using PMI as selectable gene
US11761010B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 25, 2020 |
| Grant date | Sep 19, 2023 |
| Priority date | — |
| Expiry date | Aug 29, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a soybean genetic transformation method using PMI as selectable gene, and relates to the technical field of genetic engineering. In the soybean genetic transformation method of the present invention, using the PMI gene as a selectable marker, soybean explants are infected by recombinant Agrobacterium with the PMI gene and a target gene, followed by co-culture; without recovery culture, the co-cultured explants are directly selected by a selective medium supplemented with mannose, where transformed explants with the PMI gene grow normally under selection pressure of mannose, while non-transformed explant growth is inhibited, thereby selecting successfully transformed positive plants; after shoot elongation and transplantation of the positive plants obtained, genetically transformed soybean plants are obtained successfully. Using the soybean genetic transformation method as provided by the present invention, soybeans can be genetically transformed by PMI genes derived from any species, achieving safe soybean genetic transformation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.