Recombinant Bacillus subtilis for synthesizing lacto-N-neotetraose and application thereof
US11761021B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 20, 2020 |
| Grant date | Sep 19, 2023 |
| Priority date | — |
| Expiry date | Oct 1, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/125
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure discloses recombinant Bacillus subtilis for synthesizing e lacto-N-neotetraose yield. The recombinant Bacillus subtilis is obtained by integrating two β-1,4-galactotransferase genes on a genome of a host bacterium Bacillus subtilis 168ΔamyE:P43-lacY, P43-lgtB, PxylA-comK and exogenously expressing a β-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinant Bacillus subtilis of the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation of Bacillus subtilis for producing the lacto-N-neotetraose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.