Method and sensor for detecting L-arginine

US11761922B2 · kind B2 · utility

Assignee

• CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY · CN

Inventors

• Zhong Cao · Changsha, CN
• Ningtao Zhou · Changsha, CN
• Li Zhou · Tangxia, CN
• Qin-Fan Zhu · Acton, US
• Jinglin He · Changsha, CN
• Zhongliang Xiao · Changsha, CN
• Yumin He · Changsha, CN
• Zemeng Feng · Changsha, CN
• Yulong Yin · Changsha, CN

Key dates

• Filing date: Dec 31, 2019
• Grant date: Sep 19, 2023
• Expiry date: Jun 2, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6812
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and a sensor for detecting L-arginine are provided. The method includes synthesizing ferrocene-functionalized hexadecapeptide dithiocyclopentane (FC-P16 Peptide), preparing a polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE), detecting L-Arg and other steps. The results show that the polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE) exhibits excellent electrochemical response properties to L-Arg. In 10 mmol/L phosphate-buffered saline (PBS, pH=7.4), the DPV response peak current of the polypeptide composite membrane-modified electrode has an excellent linear relationship with the L-Arg concentration of 1.0×10−13 mol/L to 1.0×10−7 mol/L, with a detection limit of 1.0×10−13 mol/L. With prominent reproducibility, repeatability and selectivity, the modified electrode has potential application in life science and nutritional health.