Ultra-sensitive chemiluminescent substrates for peroxidase

US11761964B2 · kind B2 · utility

Assignee

• CYANAGEN S.r.l. · IT

Inventors

• Leopoldo Della Ciana · Bologna, IT
• Lorenzo BIAGINI · Bologna, IT
• Thomas Paul JANSEN · Kohlenbissen, DE
• Rossana PERCIACCANTE · Granarolo dell'Emilia, IT
• Manuela VARGIOLU · Bologna, IT
• Marina Eleonora VETTRAINO · Bologna, IT

Key dates

• Filing date: Jan 22, 2020
• Grant date: Sep 19, 2023
• Expiry date: Jul 3, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/535
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase enzyme or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidizer, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from a 4-dialkylaminopyridine or an N-azole, and