Multiplex PCR amplification method for species and human individual recognition and identification of unknown biological sample suspected to be from human
US11773454B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 16, 2019 |
| Grant date | Oct 3, 2023 |
| Priority date | — |
| Expiry date | May 20, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/166
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a multiplex PCR amplification method for identifying an unknown biological sample suspected to be from a human. The method comprises the following steps: 1) acquiring an unknown biological sample suspected to be from a human and which is to be detected; 2) directly adding said unknown sample or nucleic acid extracted from said unknown sample to a premixed PCR reagent; 3) running a PCR amplification program to conduct a multiplex PCR amplification reaction; and 4) detecting and analyzing PCR amplification products, wherein the premixed PCR reagent contains primers specific to human genetic markers and primers specific to nuclear chromosomal genes of non-human species. Also provided is a multiplex PCR amplification kit for species and human individual recognition and identification of an unknown biological sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.