Peptide aptamer for specific recognition of arginine and its application

US11774455B2 · kind B2 · utility

Assignee

• INSTITUTE OF SUBTROPICAL AGRICULTURE, CHINESE ACADEMY OF SCIENCES · CN

Inventors

• Zemeng Feng · Changsha, CN
• Yumin He · Changsha, CN
• Lei DENG · Beijing, CN
• Zhong Cao · Changsha, CN
• Zhongliang Xiao · Changsha, CN
• Yulong Yin · Changsha, CN

Key dates

• Filing date: Nov 16, 2019
• Grant date: Oct 3, 2023
• Expiry date: Jun 2, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/533
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A peptide aptamer for specific recognition of arginine and its application are provided. The sequence of the peptide aptamer is shown in SEQ ID No. 1. The peptide aptamer is modified by a group that improves stability, or by a fluorescent group, an isotope and an electrochemical group that provide a detection signal, or by an affinity ligand and a mercapto. According to the computer-aided molecular docking simulation prediction, the peptide aptamer that can specifically bind to L-arginine is screened, which is verified by an isothermal titration calorimeter. The peptide aptamer has the advantages of good stability, strong binding ability, high specificity and low production cost.