Method for extracting and purifying Dendrobium officinale polysaccharides
US11780936B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 2, 2020 |
| Grant date | Oct 10, 2023 |
| Priority date | — |
| Expiry date | May 3, 2042 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A20/124
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for extracting and purifying Dendrobium officinale polysaccharides comprises following steps: (1) fully disperse Dendrobium officinale powder in pure water to obtain crude liquid; (2) removing insoluble impurities from the crude liquid through a microfiltration membrane to obtain permeate 1 and retentate 1; (3) performing macroporous ultrafiltration treatment of the permeate 1 and collect permeate 2 and retentate 2; (4) adding an aqueous solution of edible alkali metal inorganic salt to the retentate 2, fully stirring and dissolving to obtain polysaccharide crude liquid, performing macroporous ultrafiltration treatment and collecting permeate 3 and retentate 3; (5) combining the permeate 2 and permeate 3, adding the combined permeate into an electrodialysis device for desalination, and collecting dilute solution and concentrated solution; (6) performing microporous ultrafiltration treatment of the dilute solution and collect retentate 4 and permeate 4; (7) carrying out freeze-drying of the retentate 4 to obtain Dendrobium officinale polysaccharides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.