Large-scale PEI-mediated plasmid transfection
US11781102B2 · kind B2 · utility
1Cited by
3References
8Claims
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Key dates
| Filing date | Apr 25, 2017 |
| Grant date | Oct 10, 2023 |
| Priority date | — |
| Expiry date | Jul 13, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/15051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.