DNA extraction method using microwave for next generation sequencing and use thereof
US11781132B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Mar 26, 2020 |
| Grant date | Oct 10, 2023 |
| Priority date | — |
| Expiry date | Jul 10, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are a method for DNA extraction in a sample for next generation sequencing (NGS) and a method of constructing a NGS library using the extracted DNA. The method for DNA extraction includes: preparing a mixture by mixing a biological sample with a buffer; applying microwaves to the mixture; and recovering DNA. The method of constructing a NGS library includes: extracting DNA according to the method for DNA extraction; amplifying a target DNA using primers; and purifying the amplified product and subjecting the purified product to library pooling.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.