Patent · US Active

In vitro and cell based assays for measuring the activity of botulinum neurotoxins

US11788069B2 · kind B2 · utility

0Cited by
11References
27Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 10, 2022
Grant dateOct 17, 2023
Priority date—
Expiry dateMar 12, 2042

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/542
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Disclosed herein are means for the detection and characterization of neurotoxins such as botulinum neurotoxin (BoNT) or tetanus neurotoxin. The present disclosure provides methods for determining potency and activity of neurotoxins in vitro and in vivo. Also disclosed are polypeptides comprising N- and C-terminal fragments of a reporter protein that are split by a linker comprising a neurotoxin cleavage site. Cleavage of the linker by a neurotoxin decreases reporter protein activity, thereby indicating activity of the neurotoxin. Compositions and kits comprising the disclosed polypeptides, nucleic acids comprising nucleotide sequences encoding such polypeptides, and cells expressing such polypeptides are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.