Engineered polymerases with reduced sequence-specific errors

US11788075B2 · kind B2 · utility

Assignee

• Element Biosciences, Inc. · US

Inventors

• Jendrik Hentschel · San Diego, US
• Tyler Lopez · Winchester, US
• Michael Klein · Ocean City, US
• Virginia Saade · San Diego, US
• Matthew KELLINGER · San Diego, US
• Mark Ambroso · Escondido, US

Key dates

• Filing date: Jan 27, 2023
• Grant date: Oct 17, 2023
• Expiry date: Jan 27, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y207/07007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.