Padlock probe detection method

US11788119B2 · kind B2 · utility

Assignee

• Q-LINEA AB · SE

Inventors

• Camilla Russel · Uppsala, SE
• Jenny Goransson · Uppsala, SE
• Mats Gullberg · Uppsala, SE

Key dates

• Filing date: Dec 15, 2017
• Grant date: Oct 17, 2023
• Expiry date: Oct 19, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6853
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located 3′ of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs 3′ of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5′ of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.