Plant-derived exosome as well as preparation method and application thereof
US11793850B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 14, 2021 |
| Grant date | Oct 24, 2023 |
| Priority date | — |
| Expiry date | Jun 16, 2041 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L2430/32
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed a plant-derived exosome as well as a preparation method and an application thereof in preparation of drugs or scaffolds for animal tissue regeneration therapy. The preparation method includes: soaking and infiltrating any part of a natural plant with a 2-(N-morpholine) ethanesulfonic acid buffer solution; removing a supernatant; collecting a wet treated sample; refrigerating, centrifuging and extracting the sample to obtain apoplastic fluid, wherein the soaking and infiltrating method is as follows: vacuum supply is performed within 6-24 h after soaking for 2-5 times, vacuum supply time is independently 5-15 s each time, and interval time between two adjacent times of vacuum supply is independently 10 s-1 min; and centrifuging the apoplastic fluid at an ultra-high speed to obtain the plant-derived exosome, wherein ultra-high speed centrifugation conditions are as follows: centrifugal force is not lower than 100000 g, centrifugation time is 1-7 h, and a temperature is 0-4° C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.