Method of preparation of nanopore and uses thereof

US11795191B2 · kind B2 · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• GOVERNMENT OF THE UNITED STATES, AS REPRESENTED BY THE SECRETARY OF COMMERCE · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Shiv Kumar · Montgomery, US
• Chuanjuan Tao · Fort Lee, US
• Minchen Chien · Tenafly, US
• James J. Russo · New York, US
• John J. Kasianowicz · Gaithersburg, US
• Joseph W. Robertson · Washington, US

Key dates

• Filing date: Apr 1, 2019
• Grant date: Oct 24, 2023
• Expiry date: Apr 1, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/9125
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.