Methods for modulating signal intensity in interaction assays

US11796540B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Georg Kurz · Penzberg, DE
• Eloisa Lopez-Calle · Ludwigshafen, DE
• Ewelina Hegel · Mannheim, DE
• Josef Roedl · Mutterstadt, DE
• Eva Hoess · Starnberg, DE
• Joerg Kaufmann · Isenbüttel, DE
• Marisa Kirchenbauer · Dossenheim, DE

Key dates

• Filing date: Jul 26, 2019
• Grant date: Oct 24, 2023
• Expiry date: Jul 26, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4737
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure includes a method for determining an analyte in a sample in an interaction assay, including contacting a sample with an interaction modulator, wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM), aminodextran, carboxymethyldextran, Poly-(2-acrylamido-2-methyl-1-propanesulfonic acid (PAMPS), triethylamine, triethanolamine, taurine, or dodecylsulfate. The disclosure includes a method for determining an analyte in an interaction assay, including contacting the sample with an interaction modulator, wherein the interaction modulator is an enhancer of the interaction assay at low analyte concentrations and is a retarder of the interaction assay at high analyte concentrations and wherein the interaction modulator is Poly-(4-styrenesulfonic acid-co-maleic acid) (PSSM) and/or Polyacrylic acid (PAA). The disclosure further relates to a kit having a detection agent specifically detecting an analyte and at least one interaction modulator. Further, the present disclosure relates to devices relating to the methods and kits.