Method for obtaining adventitious tetraploid bud of Blumea balsamifera
US11800842B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2022 |
| Grant date | Oct 31, 2023 |
| Priority date | — |
| Expiry date | Dec 12, 2042 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H5/12
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.