Chimeric antigen receptors targeting glypican-3 or mesothelin

US11802163B2 · kind B2 · utility

Assignee

• The U.S.A., as represented by The Secretary, Department of Health and Human Services · US

Inventors

• Mitchell Ho · Urbana, US
• Nan Li · Tianjin, CN
• Dan Li · Shoreview, US

Key dates

• Filing date: Nov 7, 2018
• Grant date: Oct 31, 2023
• Expiry date: Apr 30, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16043
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Nucleic acid constructs encoding a chimeric antigen receptor (CAR) and a truncated human epidermal growth factor receptor (huEGFRt) are described. The encoded CARs include a tumor antigen-specific monoclonal antibody, such as a glypican-3 (GPC3)-specific, a GPC2-specific or a mesothelin-specific monoclonal antibody, fused to a CD8α hinge region, a CD8α transmembrane region, a 4-1BB co-stimulatory domain and a CD3ζ signaling domain. Isolated host cells, such as isolated T cells that co-express the disclosed CARs and huEGFRt are also described. T cells transduced with the disclosed CAR constructs can be used for cancer immunotherapy.