Transient silencing of ARGONAUTE1 and ARGONAUTE4 to increase recombinant protein expression in plants
US11802289B2 · kind B2 · utility
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Key dates
| Filing date | Aug 2, 2018 |
| Grant date | Oct 31, 2023 |
| Priority date | — |
| Expiry date | Aug 16, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/41
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A plant or plant cell with reduced endogenous AGO1 and AGO4 expression compared to a wild-type plant or plant cell is provided. In one embodiment, the plant or plant cell further comprises a nucleic acid sequence encoding a recombinant protein and the plant or plant cell has increased expression of the recombinant protein compared to a wild-type plant or plant cell comprising the nucleic acid sequence. Methods of reducing aglycosylation of recombinant protein produced in plants are also provided.
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