Biocontrol strain YW-1 and preparation method and application of biocontrol agent thereof

US11805782B2 · kind B2 · utility

Assignee

• HUAIYIN NORMAL UNIVERSITY · CN

Inventors

• Wei Yang · Longbeilingcun, CN
• Haixia Yan · Huai'an, CN
• Guanghui Dong · Huai'an, CN
• Yusheng Qiu · Huai'an, CN
• Yuelong Zhu · Huai'an, CN
• Zi Zhang · Huai'an, CN
• Wei Guo · Shanghai, CN
• Shimo Li · Huai'an, CN
• Lei Zhang · Beijing, CN
• Yuming Luo · Huai'an, CN

Key dates

• Filing date: Nov 10, 2021
• Grant date: Nov 7, 2023
• Expiry date: Mar 11, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A biocontrol strain YW-1, a preparation method and application of a biocontrol agent thereof are provided. The strain is Myroides odoratimimus with the preservation number of CGMCC NO. 20620. The biocontrol agent of the biocontrol strain YW-1 is obtained by the following steps: oscillating culture the biocontrol strain YW-1 in a LB culture medium at 30 Celsius degrees and 180 rpm for 12-16 hours until a total concentration of living bacteria in a bacterial suspension is 1×109-1×1010 CFU/mL; centrifuging the bacterial suspension at 6000 rpm for 10 minutes to collect bacterial cells; and diluting the bacterial cells with sterilized water to obtain a concentration of 100 CFU/milliliter. By irrigating the root of every plant with 20 milliliters of the biocontrol agent when host plants are transplanted, it can effectively prevent soil-borne diseases such as fusarium wilt of melons, bacterial wilt of tomatoes and peppers, and Phytophthora capsici.