Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
US11807857B2 · kind B2 · utility
0Cited by
237References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 24, 2015 |
| Grant date | Nov 7, 2023 |
| Priority date | — |
| Expiry date | Jun 24, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8286
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transfection of plant cells with dsRNA through foliar application encounters cuticle, cell wall and plasmalemma three major barriers. We developed cationic polymer and sugar based formulations and protocols that can effectively deliver dsRNA into plant cells resulted in gene silencing. This disclosure covers the novel methods to deliver dsRNA into plant suspension cells with ‘one step’ treatment and plant foliar cells with ‘one step’ topical application.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.