Methods and kits for using recombinant microorganisms as direct reagents in biological applications

US11807901B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Andrew Ellington · Austin, US
• Sanchita BHADRA · Austin, US
• Jared Ellefson · Austin, US
• Jimmy Gollihar · Austin, US
• Arti POTHUKUCHY · Austin, US
• Michelle BYROM · Austin, US
• Raghav Shroff · Austin, US

Key dates

• Filing date: Nov 15, 2018
• Grant date: Nov 7, 2023
• Expiry date: Feb 23, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.