Method for generating high affinity antibodies
US11808766B2 · kind B2 · utility
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47Claims
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Key dates
| Filing date | Aug 7, 2020 |
| Grant date | Nov 7, 2023 |
| Priority date | — |
| Expiry date | Apr 2, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to a method for identifying cells that express antigen-specific antibodies with a high binding affinity for a monomeric antigen. Using fluorescence activated cell sorting, cells expressing high affinity antigen-specific antibodies are selected from a population of immune cells isolated from a mammal that has been immunized with or otherwise exposed to the antigen. Nucleic acids encoding the high affinity antibodies can then be cloned into other lymphoid and non-lymphoid cells where the antibody can be expressed and from which the antibodies can be secreted.
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