Method for robust control of gene expression
US11820794B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2020 |
| Grant date | Nov 21, 2023 |
| Priority date | — |
| Expiry date | Dec 18, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/34022
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed herein include methods, compositions, and kits suitable for robust and tunable control of payload gene expression. Some embodiments provide rationally designed circuits, including miRNA-level and/or protein-level incoherent feed-forward loop circuits, that maintain the expression of a payload at an efficacious level. The circuit can comprise a promoter operably linked to a polynucleotide encoding a fusion protein comprising a payload protein, a protease, and one or more self-cleaving peptide sequences. The payload protein can comprise a degron and a cut site the protease is capable of cutting to expose the degron. The circuit can comprise a promoter operably linked to a polynucleotide comprising a payload gene, a silencer effector cassette, and one or more silencer effector binding sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.