Nucleic acid constructs and methods of using same

US11827892B2 · kind B2 · utility

Assignee

• THE STATE OF ISRAEL, MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT, AGRICULTURAL RESEARCH ORGANIZATION (ARO) (VOLCANI CENTER) · IL

Inventors

• Moshe Flaishman · Herzliya, IL
• Reut COHEN PEER · Kiryat Ono, IL
• Oded Cohen · Kiryat Tivon, IL
• Samuel Bocobza · Tel Aviv-Yafo, IL

Key dates

• Filing date: Jun 6, 2019
• Grant date: Nov 28, 2023
• Expiry date: Jun 6, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8216
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A polynucleotide comprising a nucleic acid sequence encoding an expression product of interest under a transcriptional control of a heterologous cis-acting regulatory element comprising a nucleic acid sequence at least 85% identical to SEQ ID NO: 2 (CsUbiquitin10), 4 (CsPs2), 6 (CsActin), 8 (CsTubulin), 10 (Cs EIF), 28 (metallothionein 2A), 30 (Catalase), 32 (Asparagine synthetase), 34 (60S ribosomal protein L3), 38 (40S ribosomal protein S3a), 40 (Phi-1 protein) or 44 (Receptor for activated protein kinase C) is provided. Also provided are nucleic acid constructs and cells comprising same.