Compositions and methods for nucleic acid amplification
US11827925B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 31, 2020 |
| Grant date | Nov 28, 2023 |
| Priority date | — |
| Expiry date | Nov 7, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An embodiment relates to system comprising: (i) a reaction mixture comprises: a) a target nucleic acid molecule; b) a forward primer complementary to a strand of the target nucleic acid molecule, the forward primer comprises a first molecular moiety at a 3′ end, wherein the first molecular moiety is non-complementary to the strand of the target nucleic acid molecule; c) a reverse primer complementary to a complementary sequence of the strand of the target nucleic acid molecule, the reverse primer comprises a second molecular moiety at a 3′ end, wherein the second molecular moiety is non-complementary to the complementary sequence of the strand of the target nucleic acid molecule; d) a polymerase with 3′-5′ exonuclease activity; and (ii a device suitable of detecting an amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.