Methods and systems for single cell gene profiling

US11827936B2 · kind B2 · utility

Assignee

• FLUENT BIOSCIENCES INC. · US

Inventors

• Kristina Fontanez · Arlington, US
• Robert Meltzer · Long Valley, US
• Yi Xue · Shrewsbury, US
• Christopher D'amato · Wellesley, US
• Sepehr Kiani · Cambridge, US

Key dates

• Filing date: Jan 12, 2021
• Grant date: Nov 28, 2023
• Expiry date: Jan 12, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.