Multichannel excitation and emission for miniaturized, planar fluorescence activated cell sorting
US11828697B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 2, 2017 |
| Grant date | Nov 28, 2023 |
| Priority date | — |
| Expiry date | Apr 8, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/068
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for fluorescence activated cell sorting includes at least two excitation lasers having different orientations relative to an objective such that light from the at least two lasers passes through the objective and intersects a fluidic channel at different positions within an interrogation region. The fluidic channel directs a flow of a plurality of fluorescently labeled particles through the interrogation region. The system further includes at least one detector and at least one optical element that directs light emitted from the plurality of fluorescently labeled particles and transmitted through the objective to the at least one detector. The system may further include optics for generating and detecting side and forward scattered light. Methods for operating example systems to collect fluorescent, side scattered and forward scattered light from a plurality of particles are also described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.