Method for detecting aflatoxin B1 based on fluorescent copper nanoparticles
US11828761B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 27, 2022 |
| Grant date | Nov 28, 2023 |
| Priority date | — |
| Expiry date | May 27, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/38
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for detecting aflatoxin B1 based on fluorescent copper nanoparticles, belonging to the technical fields of analytical chemistry, materials science and nano biosensing. In the disclosure, β-CD@DNA-Cu NMs are prepared by using Y-shaped DNA as a template, ascorbic acid as a reducing agent and β-CD as a fluorescence stabilizing and enhancing agent. Then, a ratiometric fluorescent probe is constructed based on the β-CD@DNA-Cu NMs. Finally, the detection of AFB1 with high sensitivity, high selectivity and high accuracy is achieved by using the fluorescent probe. According to the method of the disclosure, in linear ranges of 0.03-10 ppb and 10-18 ppb, a ratio value of I433 nm/I650 nm and a concentration of AFB1 exhibit a good linear relationship respectively, and a limit of detection is 0.012 ppb (S/N=3). Metal ions Ca2+ may be replaced with Yb3+, Y3+, Er3+ and Pt2+, which are also suitable for increasing sensitivity of AFB1 in rice.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.