Oligonucleotide-comprising cellular component-binding reagents and methods of using the same

US11834715B2 · kind B2 · utility

Assignee

• Becton, Dickinson and Company · US

Inventors

• Margaret Nakamoto · Palo Alto, US
• Eleen Shum · San Jose, US
• Nidhanjali Bansal · Menlo Park, US
• Christina Chang · Menlo Park, US

Key dates

• Filing date: Jan 21, 2020
• Grant date: Dec 5, 2023
• Expiry date: Feb 11, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).