Multispectral sample imaging

US11835463B2 · kind B2 · utility

Assignee

• Akoya Biosciences, Inc. · US

Inventors

• Peter J. Miller · Broomfield, US
• Kent Johnson · Bethesda, US
• Carla Coltharp · Waltham, US

Key dates

• Filing date: Apr 14, 2022
• Grant date: Dec 5, 2023
• Expiry date: Apr 14, 2042

Classification

• Technology area (CPC —): General

Abstract

The disclosure features methods that include exposing a biological sample to illumination light and measuring light emission from the sample to obtain N sample images, where each sample image corresponds to a different combination of a wavelength band of the illumination light and one or more wavelength bands of the light emission, where the one or more wavelength bands of the light emission define a wavelength range, and where N>1, and exposing the sample to illumination light in a background excitation band and measuring light emission from the sample in a background spectral band to obtain a background image of the sample, where the background spectral band corresponds to a wavelength within the wavelength range.