Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted DNA using cell endogenous DNA modifying enzyme, and molecular complex used therein
US11845953B2 · kind B2 · utility
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Key dates
| Filing date | Mar 20, 2018 |
| Grant date | Dec 19, 2023 |
| Priority date | — |
| Expiry date | Apr 20, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.