Scatter labeled imaging of microvasculature in excised tissue (SLIME)

US11845955B2 · kind B2 · utility

Assignees

• CASE WESTERN RESERVE UNIVERSITY · US
• NATIONAL INSTITUTES OF HEALTH (NIH) · US
• U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS) · US
• U.S. GOVERNMENT NIH DIVISION OF EXTRAMURAL INVENTIONS AND TECHNOLOGY RESOURCES (DEITR) · US

Inventors

• Michael Jenkins · Cleveland, US
• Yehe Liu · Cleveland, US

Key dates

• Filing date: Aug 1, 2022
• Grant date: Dec 19, 2023
• Expiry date: Aug 1, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/30024
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed “scatter labeled imaging of microvasculature in excised tissue” (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample. The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent and subsequently imaged.