Detecting and characterizing a microorganism

US11845978B2 · kind B2 · utility

Assignee

• Q-LINEA AB · SE

Inventors

• Jonas Jarvius · Uppsala, SE
• Anders Lind · Tärnsjö, SE
• Henrik Soderstrom · Knivsta, SE
• Jan Grawe · Uppsala, SE

Key dates

• Filing date: Apr 21, 2017
• Grant date: Dec 19, 2023
• Expiry date: Feb 19, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for detecting and characterising a microorganism in a clinical sample includes introducing the clinical sample to a first culture vessel and simultaneously transporting and incubating the clinical sample in a thermally insulated compartment of a sealable container. A test aliquot is removed from the sample and the remainder of the sample is further cultured. DNA is removed from the test aliquot and nucleic acid tests are performed on it to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. One or more nucleic acid probes or primers are used to perform the nucleic acid tests. It is detected whether or not the probes or primers have hybridised to DNA or the primers have been extended. If a microorganism is identified, then an antimicrobial susceptibility test is performed on the cultured clinical sample.