Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
US11845985B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 10, 2022 |
| Grant date | Dec 19, 2023 |
| Priority date | — |
| Expiry date | May 10, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and/or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.