Patent · US Active

Organelle-targeted genetically-encoded voltage indicators and methods of use thereof

US11846628B2 · kind B2 · utility

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2References
18Claims
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Key dates

Filing dateAug 23, 2022
Grant dateDec 19, 2023
Priority date—
Expiry dateAug 23, 2042

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2319/60
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

As described herein, a hybrid voltage sensor genetically-encoded voltage indicator (GEVI) for mitochondria or endoplasmic reticulum includes a transmembrane domain, and a fluorescent protein, wherein a terminus of the transmembrane domain and a terminus of the fluorescent protein are covalently linked directly or by a linker comprising 1 to 20 amino acids, and wherein the transmembrane domain comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a peptide with greater than 85%, 90%, 95% or 98% identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4. Also described are expression vectors, expression cassettes, and organelle membranes, as well as methods of determining the voltage across an organelle using the GEVIs.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.