InDel marker genotype database of Malus and use in identity fingerprinting of germplasm resources

US11851718B2 · kind B2 · utility

Assignee

• China Agricultural University · CN

Inventors

• Xinzhong Zhang · Mason, US
• Xuan Wang · Xuzhou, CN
• Fei Shen · Shanghai, CN
• Ruiting CHEN · Beijing, CN
• Zhenhai Han · Beijing, CN
• Yi Wang · Lanzhou, CN
• Ting Wu · Ningbo City, CN
• Xuefeng Xu · Zhejiang, CN
• Wei Li · Beijing, CN
• Changpeng Qiu · Beijing, CN
• Xi Zhang · Shandong, CN

Key dates

• Filing date: Dec 29, 2020
• Grant date: Dec 26, 2023
• Expiry date: Jul 19, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure provides an InDel marker genotype database of whole genome of Malus and use in specific identification of germplasm resources thereof, belonging to the technical field of molecular biology. The constructing method of the genotype database comprises: genotyping InDel markers of genomic DNA samples of Malus germplasm resources by using primer pairs and multiplex PCR; recording one line data for each Malus germplasm resource according to the genotype of InDel marker; collecting all germplasm resources records to obtain an InDel marker genotype database of the whole genome of Malus. The whole genome InDel marker genotype database of Malus provided by the disclosure can identify the specificity of apple germplasm simply, efficiently and stably, thus overcoming the limitation that DUS test is easily influenced by environment, and providing a powerful tool for the protection of new apple varieties and the management of germplasm resources.