Multi-signal fluorescent probe for early diagnosis of tumors, and preparation and use thereof

US11857644B2 · kind B2 · utility

Assignees

• Hunan Cancer Hospital · CN
• Hunan Normal University · CN

Inventors

• Huijun ZHOU · Changsha, CN
• Zhenyang Liu · Beijing, CN
• Pan Chen · Shanghai, CN
• Xiaming Zhang · Changsha, CN
• Peng Yin · Ithaca, US
• Ting Li · Beijing, CN

Key dates

• Filing date: Mar 24, 2023
• Grant date: Jan 2, 2024
• Expiry date: May 16, 2043

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A multi-signal fluorescent probe, represented by: A method for preparing the multi-signal fluorescent probe includes: (a) adding 2-methoxyphenothiazine and ethyl iodide into a mixture of dichloromethane (DCM) and acetonitrile followed by a first reaction and a first post-treatment to obtain 10-ethyl-2-methoxy-10H-phenothiazine; (b) adding boron tribromide into the 10-ethyl-2-methoxy-10H-phenothiazine under an inert gas followed by a second reaction under an ice bath and a second post-treatment to obtain 10-ethyl-10H-phenothiazin-2-ol; and (c) mixing the 10-ethyl-10H-phenothiazin-2-ol, malonic acid, zinc chloride and phosphorus oxychloride followed by a third reaction and a third post-treatment to obtain the multi-signal fluorescent probe. A use of the multi-signal fluorescent probe in the detection of intracellular ONOO− and Na2S2 is also provided.